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在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后

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Developmental Biology

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JoVE Journal Developmental Biology

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Please note that all translations are automatically generated. Click here for the English version.

在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后

DOI:

10.3791/57290-v

•

09:38 min

•

February 20, 2018

•

Renee Wei-Yan Chow*^1,2,3,4, Paola Lamperti*^1,2,3,4, Emily Steed^2,3,4, Francesco Boselli^2,3,4, Julien Vermot^2,3,4

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, ²UMR7104,Centre National de la Recherche Scientifique, ³U964,Institut National de la Santé et de la Recherche Médicale, ⁴Université de Strasbourg

Chapters

00:04Title
00:53Preparing Molds and Mounting Agarose and Embedding Embryos
03:31Photoconversion
05:12Unmounting Photoconverted Embryos
06:20Imaging Photoconverted Cells at Later Embryonic Stages
08:05Results: Photoconversion of Kaede Fluorescent Protein in Zebrafish Embryonic Endocardial Cells
09:01Conclusion

Summary

Automatic Translation

该协议描述了在活斑马鱼胚胎的心内膜细胞中 photoconversion 枫荧光蛋白的方法, 能够在房室管和房室心脏瓣膜发育过程中跟踪心内膜细胞。.

Tags

Cell Photoconversion Zebrafish Embryo Atrioventricular Canal Heart Valve Formation Cardiac Development Mutants Time-lapse Microscopy Agarose Mounting Medium Low Melting Point Agarose Tricaine BDM

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