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A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons
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Neuroscience
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JoVE Journal Neuroscience
A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons

A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons

DOI:
10.3791/57202-v

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09:58 min

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April 30, 2018

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, ,
1Molecular, Cellular, and Developmental Biology Program,The Ohio State University, 2Department of Biological Chemistry and Pharmacology,The Ohio State University, 3Biogen

Chapters

  • 00:05Title
  • 00:55Coverslip Preparation
  • 02:43Dissociation and Culture of Hippocampal Neurons
  • 06:41Setting Up the Puffing Pipette Apparatus
  • 08:27Results: Representative Images of Varicosity Formation Following Puffing
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a physiologically relevant, pressurized fluid approach for rapid and reversible induction of varicosities in neurons.

Tags

Keywords: Microbiomechanical SystemVaricosity FormationAxon RecoveryCentral NeuronBrain InjuryNeuronal PlasticityDrug ScreeningTraumatic Brain InjuryHippocampal Neuron CultureProtease EnzymePlating MediumSubcellular MorphologyMechanical Stress

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