Journal
/
神经科学
/
A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons

A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons

DOI:
10.3791/57202-v

•

09:58 min

•

April 30, 2018

•

, ,
1Molecular, Cellular, and Developmental Biology Program,The Ohio State University, 2Department of Biological Chemistry and Pharmacology,The Ohio State University, 3Biogen

Chapters

  • 00:05Title
  • 00:55Coverslip Preparation
  • 02:43Dissociation and Culture of Hippocampal Neurons
  • 06:41Setting Up the Puffing Pipette Apparatus
  • 08:27Results: Representative Images of Varicosity Formation Following Puffing
  • 09:09Conclusion

Summary

自动翻译

自动翻译

This protocol describes a physiologically relevant, pressurized fluid approach for rapid and reversible induction of varicosities in neurons.

Tags

Keywords: Microbiomechanical SystemVaricosity FormationAxon RecoveryCentral NeuronBrain InjuryNeuronal PlasticityDrug ScreeningTraumatic Brain InjuryHippocampal Neuron CultureProtease EnzymePlating MediumSubcellular MorphologyMechanical Stress

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/57202?article_type=v

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
check_url/cn/57202?article_type=v

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics
check_url/cn/57202?article_type=v

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
check_url/cn/57202?article_type=v

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
check_url/cn/57202?article_type=v

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds
check_url/cn/57202?article_type=v

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
check_url/cn/57202?article_type=v

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
check_url/cn/57202?article_type=v

Laser Nanosurgery of Cerebellar Axons In Vivo
check_url/cn/57202?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/cn/57202?article_type=v

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

Read Article