/
/
Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,309 Views
•
08:24 min
•
February 27, 2018
DOI:
Summary
Automatically generated
The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.
Read Article
Cite this Article
Guess, T., Lai, H., Smith, S. E., Sircy, L., Cunningham, K., Nelson, D. E., McClelland, E. E. Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans. J. Vis. Exp. (132), e57171, doi:10.3791/57171 (2018).
Copy