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Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
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February 27, 2018
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The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.
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Guess, T., Lai, H., Smith, S. E., Sircy, L., Cunningham, K., Nelson, D. E., McClelland, E. E. Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans. J. Vis. Exp. (132), e57171, doi:10.3791/57171 (2018).
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