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水溶性と不溶性の Huntingtin 種の解像度の分別

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

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水溶性と不溶性の Huntingtin 種の解像度の分別

9,309 Views

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07:08 min

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February 27, 2018

DOI:

10.3791/57082-v

DOI:

10.3791/57082-v

•

07:08 min

February 27, 2018

•

5 Views

Joseph Ochaba^1,2, Eva L. Morozko^2,3, Jacqueline G. O’Rourke⁴, Leslie M. Thompson^1,2,3

¹Department of Psychiatry and Human Behavior,University of California Irvine, ²UCI MIND,University of California Irvine, ³Department of Neurobiology and Behavior,University of California Irvine, ⁴Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center

Summary

不溶解性および溶ける変異ハンチンチン種マウス脳と細胞培養からの分別の方法を説明します。記載されているメソッドは、特性評価および huntingtin 蛋白質フラックスと病気の発症と摂動存在下での蛋白質の恒常性の分析にエイズの定量化の便利

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