Journal
/
Biochimie
/
Fractionation for Resolution of Soluble and Insoluble Huntingtin Species
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

DOI:
10.3791/57082-v

•

07:08 min

•

February 27, 2018

•

, , ,
1Department of Psychiatry and Human Behavior,University of California Irvine, 2UCI MIND,University of California Irvine, 3Department of Neurobiology and Behavior,University of California Irvine, 4Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center

Chapitres

  • 00:04Titre
  • 00:51Soluble/Insoluble Fractionation Protocol
  • 03:58Western Blot and Quantification
  • 04:31Results: Representative Results for Soluble and Insoluble Fractionations and Analyses
  • 06:18Conclusion

Summary

Traduction automatique

Traduction automatique

A method is described for fractionation of insoluble and soluble mutant huntingtin species from mouse brain and cell culture. The method described is useful for characterization and quantification of huntingtin protein flux and aids in analyzing protein homeostasis in disease pathogenesis and in the presence of perturbations

Tags

FractionationHuntingtinSolubleInsolubleProtein HomeostasisHuntington’s DiseaseNeurodegenerative DiseaseProtein MisfoldingLysis BufferProtease InhibitorsDounce HomogenizationCell LysateCentrifugationSupernatantPellet

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Read Article