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Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development
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Developmental Biology
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JoVE Journal Developmental Biology
Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

English

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7,788 Views

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10:30 min

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June 12, 2018

DOI:

10.3791/57068-v

DOI:
10.3791/57068-v

•

10:30 min
June 12, 2018

•

3 Views
, , ,
1Faculty of Life and Environmental Sciences, Department of Biotechnology,University of Yamanashi, 2Department of Integrated Bioscience, Graduate School of Frontier Sciences,University of Tokyo, 3Advanced Biotechnology Center,University of Yamanashi

Summary

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Chromatin looseness appears to be involved in the developmental potential of blastomeres. However, it is not known whether chromatin looseness can be used as a reliable index for the developmental potential for embryos. Here, an experimental system in which chromatin looseness-evaluated zygotes can develop to full term has been described.

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Cite this Article

Ooga, M., Funaya, S., Aoki, F., Wakayama, T. Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development. J. Vis. Exp. (136), e57068, doi:10.3791/57068 (2018).

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