Journal
/
Biologie du développement
/
Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

7,788 Views

•

10:30 min

•

June 12, 2018

DOI:

10.3791/57068-v

DOI:
10.3791/57068-v

•

10:30 min
June 12, 2018

•

3 Views
, , ,
1Faculty of Life and Environmental Sciences, Department of Biotechnology,University of Yamanashi, 2Department of Integrated Bioscience, Graduate School of Frontier Sciences,University of Tokyo, 3Advanced Biotechnology Center,University of Yamanashi

Summary

Automatically generated

Chromatin looseness appears to be involved in the developmental potential of blastomeres. However, it is not known whether chromatin looseness can be used as a reliable index for the developmental potential for embryos. Here, an experimental system in which chromatin looseness-evaluated zygotes can develop to full term has been described.

Explore More Videos

Zygotic FRAPZFRAPChromatin LoosenessEmbryonic DevelopmentEGFP-H2BMRNA InjectionMicroinjectionConfocal MicroscopyPhoto-bleaching

Read Article

Cite this Article

Ooga, M., Funaya, S., Aoki, F., Wakayama, T. Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development. J. Vis. Exp. (136), e57068, doi:10.3791/57068 (2018).

Download .ris file

Copy

Share Video

.

Copy