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Amplificazione isotermica multiplex basato su piattaforma diagnostica per rilevare Zika, Chikungunya e Dengue 1
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Biochemistry
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JoVE Journal Biochemistry
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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Amplificazione isotermica multiplex basato su piattaforma diagnostica per rilevare Zika, Chikungunya e Dengue 1

italiano

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14,007 Views

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06:18 min

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March 13, 2018

DOI:

10.3791/57051-v

DOI:
10.3791/57051-v

•

06:18 min
March 13, 2018

•

13 Views
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1Foundation for Applied Molecular Evolution (FfAME), 2Florida Medical Entomology Laboratory,University of Florida, 3Firebird Biomolecular Sciences LLC

Summary

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Corrente multiplexed diagnostica per rilevare virus dengue, chikungunya e Zika richiedono preparazione campioni complessi e costosi strumentazione e sono difficili da utilizzare in ambienti di risorse in esaurimento. Vi mostriamo una diagnostica che utilizza amplificazione isotermica con sonde displaceable strand mirate per rilevare e distinguere questi virus con alta sensibilità e specificità.

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Cite this Article

Yaren, O., Alto, B. W., Bradley, K. M., Moussatche, P., Glushakova, L., Benner, S. A. Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1. J. Vis. Exp. (133), e57051, doi:10.3791/57051 (2018).

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