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Proliferazione e differenziazione dei precursori mieloidi murino 32D/G-CSF-R cellule
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Developmental Biology
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JoVE Journal Developmental Biology
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Proliferazione e differenziazione dei precursori mieloidi murino 32D/G-CSF-R cellule

italiano

Automatically Generated

9,711 Views

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10:21 min

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February 21, 2018

DOI:

10.3791/57033-v

DOI:
10.3791/57033-v

•

10:21 min
February 21, 2018

•

3 Views
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1Department of Hemato-Oncology,Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine,Charles University

Summary

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Qui vengono presentati protocolli dettagliati per coltura la linea 32D/G-CSF-R cellula precursore mieloide murino, eseguendo le infezioni virali e svolgere le analisi di proliferazione e differenziazione. Questa linea cellulare è adatta per lo studio dello sviluppo delle cellule mieloidi e il ruolo dei geni di interesse in crescita delle cellule mieloidi e differenziazione neutrophilic.

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Zjablovskaja, P., Danek, P., Kardosova, M., Alberich-Jorda, M. Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells. J. Vis. Exp. (132), e57033, doi:10.3791/57033 (2018).

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