03:28Tritation of Virus Containing a Puromycin Reporter
05:04Transduction of 32D/G-CSF-R Cells
06:1032D/G-CSF-R Cell Differentiation Assay
07:32Results: Study of Proliferation and Differentiation of 32D/G-CSF-R Cells in Interleukin-3 and Granulocyte Colony Stimulating Factor Containing Media
Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.