Journal
/
Immunology and Infection
/
Visualizando a actina e microtúbulos citoesqueleto na célula B sinapse imune usando microscopia de depleção (STED) emissão estimulada
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy

Visualizando a actina e microtúbulos citoesqueleto na célula B sinapse imune usando microscopia de depleção (STED) emissão estimulada

13,388 Views

•

11:00 min

•

April 09, 2018

DOI:

10.3791/57028-v

DOI:
10.3791/57028-v

•

11:00 min
April 09, 2018

•

6 Views
, ,
1Department of Microbiology and Immunology,University of British Columbia

Tags

ActinMicrotubuleCytoskeletonB-cellImmune SynapseSTED MicroscopyCytoskeleton OrganizationCytoskeleton ReorganizationImmune Cell ActivationFluorophoresA20 B-lymphoma CellsTransfectionGoat Anti-mouse Immunoglobulin G AntibodyCoverslips

Read Article

Cite this Article

Wang, J. C., Bolger-Munro, M., Gold, M. R. Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy. J. Vis. Exp. (134), e57028, doi:10.3791/57028 (2018).

Download .ris file

Copy

Share Video

.

Copy