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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy
 

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy

Article DOI: 10.3791/57028-v • 11:00 min • April 9th, 2018
April 9th, 2018 •
1, 1, 1
1Department of Microbiology and Immunology, University of British Columbia

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We present a protocol for using STED microscopy to simultaneously image actin structures, microtubules, and microtubule plus-end binding proteins in B cells that have spread on coverslips coated with antibodies to the B-cell receptor, a model for the initial phase of immune synapse formation.

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Actin Microtubule Cytoskeleton B-cell Immune Synapse Stimulated Emission Depletion Microscopy STED Microscopy Structure Organization Spatial Relationships Cytoskeleton Organization B-cell Activation Multi-color STED Microscopy Nanometer Scale Resolution Immune Synapse Formation Natural Killer Cells T-cells Microscope Settings Fluorophores Centrifugation Transfection Reagent Plasma DNA
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