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非正規のアミノ酸の選択圧による 'ゴールデン' 蛍光と質量分析法と蛍光タンパク質解析工学
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Engineering ‘Golden’ Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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非正規のアミノ酸の選択圧による 'ゴールデン' 蛍光と質量分析法と蛍光タンパク質解析工学

日本語

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11,640 Views

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11:51 min

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April 27, 2018

DOI:

10.3791/57017-v

DOI:
10.3791/57017-v

•

11:51 min
April 27, 2018

•

1 Views
, , , , , ,
1Institute of Chemistry L 1, Department of Biocatalysis,Technical University of Berlin, 2Institute of Chemistry PC 14, Department of Bioenergetics,Technical University of Berlin, 3Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences,Technical University of Berlin

Summary

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合成生物学は、非正規のアミノ酸の co-translational の挿入を使用して前例のないプロパティとタンパク質工学を可能です。ここでは、我々 は選択圧定款 (SPI) 経由のエシェリヒア属大腸菌の「ゴールド」蛍光タンパク質 (GdFP) と呼ばれる、蛍光分光特性を持つ型 GFP 蛍光のスペクトル赤シフト変形を生成する方法を提案しました。

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Baumann, T., Schmitt, F., Pelzer, A., Spiering, V. J., Freiherr von Sass, G. J., Friedrich, T., Budisa, N. Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence. J. Vis. Exp. (134), e57017, doi:10.3791/57017 (2018).

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