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用质谱和荧光法研究非规范氨基酸和蛋白质分析的选择性压力下工程 "金" 荧光
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Engineering ‘Golden’ Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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用质谱和荧光法研究非规范氨基酸和蛋白质分析的选择性压力下工程 "金" 荧光

English

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11,604 Views

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11:51 min

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April 27, 2018

DOI:

10.3791/57017-v

DOI:
10.3791/57017-v

•

11:51 min
April 27, 2018

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1 Views
, , , , , ,
1Institute of Chemistry L 1, Department of Biocatalysis,Technical University of Berlin, 2Institute of Chemistry PC 14, Department of Bioenergetics,Technical University of Berlin, 3Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences,Technical University of Berlin

Summary

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合成生物学使蛋白质的工程具有空前的特性, 使用非规范氨基酸的共平移插入。在这里, 我们介绍了如何一个光谱红色转移变种的 GFP 型荧光与新的荧光光谱性质, 称为 "金" 荧光蛋白 (GdFP), 是产生于大肠杆菌通过选择性压力成立 (SPI)。

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Baumann, T., Schmitt, F., Pelzer, A., Spiering, V. J., Freiherr von Sass, G. J., Friedrich, T., Budisa, N. Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence. J. Vis. Exp. (134), e57017, doi:10.3791/57017 (2018).

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