Journal
/
Neuroscience
/
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

English

Automatically Generated

11,267 Views

•

11:26 min

•

February 07, 2018

DOI:

10.3791/56911-v

DOI:
10.3791/56911-v

•

11:26 min
February 07, 2018

•

10 Views
, , , , ,
1Neuroscience Program,University of Miami School of Medicine, 2Department of Biology,University of Miami

Summary

Automatically generated

This protocol describes the use of genetically encoded Ca2+ reporters to record changes in neural activity in behaving Caenorhabditis elegans worms.  

Explore More Videos

Ratiometric Calcium ImagingCaenorhabditis ElegansHSN NeuronBehavioral NeuroscienceGCaMP5MCherryNematode Growth MediumOP50 BacteriaVulvaInverted MicroscopeInfrared FilterVideo AnalysisStage XY Stage Control

Read Article

Cite this Article

Ravi, B., Nassar, L. M., Kopchock III, R. J., Dhakal, P., Scheetz, M., Collins, K. M. Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans. J. Vis. Exp. (132), e56911, doi:10.3791/56911 (2018).

Download .ris file

Copy

Share Video

.

Copy