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Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

11,262 Views

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11:26 min

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February 07, 2018

DOI:

10.3791/56911-v

DOI:
10.3791/56911-v

•

11:26 min
February 07, 2018

•

10 Views
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1Neuroscience Program,University of Miami School of Medicine, 2Department of Biology,University of Miami

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This protocol describes the use of genetically encoded Ca2+ reporters to record changes in neural activity in behaving Caenorhabditis elegans worms.  

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Ravi, B., Nassar, L. M., Kopchock III, R. J., Dhakal, P., Scheetz, M., Collins, K. M. Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans. J. Vis. Exp. (132), e56911, doi:10.3791/56911 (2018).

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