Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Metabolic Labeling and Profiling of Transfer RNAs …
JoVE Journal
Genetics

This Contenuto is Open Access.

JoVE Journal Genetics
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
 

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Article DOI: 10.3791/56898-v • 10:56 min • January 16th, 2018
January 16th, 2018 •
*1, *1, *1, 1, 2, 2, 3, 1
1Laboratory of tRNA Biology, Department of Biology, College of Charleston, 2Department of Biochemistry and Molecular Biology, MUSC, 3Department of Biology, College of Charleston
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.

Tags

Metabolic Labeling Profiling Transfer RNAs Macroarrays In Vivo Labeling Macroarray Analysis TRNA Levels Regulatory Functions Fluctuation Of TRNA Expression Gene Translation Protein Synthesis Quantification Of TRNA Levels Laboratory-grown Organisms Mycobacterium Smegmatis Radioprotection Practices Phosphorus-32-labeled Orthophosphate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter