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January 29, 2018
DOI:
1Department of Pathology, Immunology and Experimental Medicine,University of Florida, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,University of Florida, 4Department of Biomedical Engineering, College of Engineering,University of Florida, 5Department of Pediatrics, College of Medicine,University of Florida
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Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.
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Butterworth, E., Dickerson, W., Vijay, V., Weitzel, K., Cooper, J., Atkinson, E. W., Coleman, J. E., Otto, K. J., Campbell-Thompson, M. High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network. J. Vis. Exp. (131), e56859, doi:10.3791/56859 (2018).
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