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High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
1Department of Pathology, Immunology and Experimental Medicine,Florida Üniversitesi, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,Florida Üniversitesi, 4Department of Biomedical Engineering, College of Engineering,Florida Üniversitesi, 5Department of Pediatrics, College of Medicine,Florida Üniversitesi
Bölümler
- 00:04Başlık
- 00:35Pancreas Fixation and Hydrogel Embedding
- 03:37Tissue Clearing
- 04:38Immunofluorescence Staining
- 07:03Mounting Samples for Imaging
- 08:30Results: Human Neuro-insular Network and 3D Schwann Cell Visualization
- 09:35Conclusion
Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.
