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バインドの動的・電気泳動光散乱による解析 DNA 磁性粒子
JoVE Journal
Chemistry
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JoVE Journal Chemistry
DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
DOI:

10:35 min

November 09, 2017

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Chapters

  • 00:05Title
  • 00:49Synthesis of Magnetic Nanoparticles (MNPs)
  • 03:07Chemical Modification of Magnetic Nanoparticles
  • 05:53Magnetic Particle Size Analysis Using DLS
  • 06:46Testing Efficiency of DNA Isolation Using Quantitative PCR
  • 07:58Magnetic Particle Zeta Potential Analysis Using DLS
  • 08:37Results: Zeta Potential of Representative Magnetic Particles Over Time in Presence and Absence of DNA
  • 09:57Conclusion

Summary

Automatic Translation

このプロトコルでは、磁性粒子の合成と DNA 結合の動的・電気泳動光散乱法による評価について説明します。粒子サイズ、彼らの多分散性の変化の監視に焦点を当てこのメソッドとを担う粒子表面のゼータ電位の主要な DNA など材料の結合の役割。

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