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Imagerie de Mycobacterium tuberculosis chez la souris avec la journaliste Enzyme Fluorescence
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
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Imagerie de Mycobacterium tuberculosis chez la souris avec la journaliste Enzyme Fluorescence

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7,092 Views

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10:06 min

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February 26, 2018

DOI:

10.3791/56801-v

DOI:
10.3791/56801-v

•

10:06 min
February 26, 2018

•

2 Views
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1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Summary

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Nous décrivons l’imagerie optique des souris infectées par Mycobacterium tuberculosis (M. tuberculosis) utilisant journaliste enzyme fluorescence (REF). Ce protocole facilite la détection sensible et spécifique de M. tuberculosis dans des modèles animaux précliniques pour recherche de vaccin, la thérapeutique et la pathogenèse.

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Cite this Article

Sharan, R., Yang, H., Sule, P., Cirillo, J. D. Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence. J. Vis. Exp. (132), e56801, doi:10.3791/56801 (2018).

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