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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

7,092 Views

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10:06 min

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February 26, 2018

DOI:

10.3791/56801-v

DOI:
10.3791/56801-v

•

10:06 min
February 26, 2018

•

2 Views
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1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Summary

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We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine research.

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Cite this Article

Sharan, R., Yang, H., Sule, P., Cirillo, J. D. Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence. J. Vis. Exp. (132), e56801, doi:10.3791/56801 (2018).

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