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Developmental Biology

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小鼠骨髓基质细胞与骨破骨祖的分离、培养及分化

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Developmental Biology

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JoVE Journal Developmental Biology

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

Please note that all translations are automatically generated. Click here for the English version.

小鼠骨髓基质细胞与骨破骨祖的分离、培养及分化

DOI:

10.3791/56750-v

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08:07 min

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January 06, 2018

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David E. Maridas, Elizabeth Rendina-Ruedy, Phuong T. Le, Clifford J. Rosen

¹Maine Medical Center Research Institute

Chapters

00:05Title
00:41Collection Tube Preparation and Bone Harvest
02:52Total Bone Marrow Cell (BMC) and Split BMC Population Plating and Culture
04:45Bone Marrow Stem Cell (BMSC) Differentiation
06:07Results: Representative BMSC Differentiation
07:27Conclusion

Summary

Automatic Translation

本文介绍了从小鼠长骨分离分化骨髓基质细胞和造血干细胞的方法。两种不同的协议, 产生不同的细胞群体, 适合扩展和分化成成骨细胞, 脂肪和破骨。

Tags

Keywords: Bone Marrow Stromal Cells BMSCs Bone And Adipocyte Progenitor Cells Bone Harvest Bone Marrow Isolation Cell Culture Cell Differentiation Osteoclast Progenitors Mouse Visual Demonstration

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