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Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes
1Translational Experimental Pediatrics, Experimental Pulmonology, Department of Pediatrics and Adolescent Medicine,University of Cologne, 2Department of Pediatrics and Adolescent Medicine,University of Cologne, 3Division of Experimental Pediatrics and Metabolism, University Children’s Hospital,Heinrich Heine University Düsseldorf
Chapters
- 00:04Title
- 01:33Intraperitoneal Glucose Tolerance Test (ipGTT) and Lung Function Analysis
- 04:44Lung Isolation
- 06:02Results: Representative ipGTT and Lung Function Analyses
- 07:07Conclusion
The incidence of obesity is rising and increases the risk of chronic lung diseases. To establish the underlying mechanisms and preventive strategies, well-defined animal models are needed. Here, we provide three methods (glucose-tolerance-test, body plethysmography, and lung fixation) to study the effect of obesity on pulmonary outcomes in mice.
Tags
Intraperitoneal Glucose Tolerance TestLung Function MeasurementLung FixationObesityImpaired MetabolismPulmonary OutcomesRespiratory ResearchMetabolic DisordersLung StructureLung FunctionChronic Lung DiseasesTherapeutic ApproachesCOPDAsthmaFasted Blood GlucoseGlucose InjectionLung Function AssessmentAnesthesiaAnimal Models
