Journal
/
/
Immuno-fluorescerende mærkning af mikrotubuli og Centrosomal proteiner i Ex Vivo tarmens væv og 3D In Vitro tarm Organoids
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
DOI:

09:51 min

December 13, 2017

, , , ,

Chapters

  • 00:05Title
  • 01:41Isolation of Intestinal Organoids from Basement Matrix Domes in 24-well Plates
  • 03:20Fixation of Isolated Organoids
  • 04:22Blocking, and Primary and Secondary Antibody Incubation
  • 06:41Mounting Isolated Organoids
  • 07:39Results: Isolation and Immunolabeling of Intestinal Organoids
  • 08:55Conclusion

Summary

Automatic Translation

Vi præsenterer protokoller til isolation af intestinal 3D strukturer fra væv i vivo og in vitro- kælderen matrix indlejret organoids, og detaljer forskellige fiksering og farvning protokoller optimeret til immuno-mærkning af mikrotubulus, centrosomal og junctional proteiner samt som celle markører herunder stamceller protein Lgr5.

Related Videos

Read Article