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Biologia do Desenvolvimento
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Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

14,831 Views

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09:51 min

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December 13, 2017

DOI:

10.3791/56662-v

DOI:
10.3791/56662-v

•

09:51 min
December 13, 2017

•

14 Views
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1School of Biological Sciences,University of East Anglia, 2Norwich Medical School,University of East Anglia

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3D Intestinal OrganoidsImmunofluorescent LabelingMicrotubulesCentrosomal ProteinsFixationIsolationImmunostaining

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Goldspink, D. A., Matthews, Z. J., Lund, E. K., Wileman, T., Mogensen, M. M. Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids. J. Vis. Exp. (130), e56662, doi:10.3791/56662 (2017).

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