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分離とシロイヌナズナ由来のミトコンドリアの呼吸測定
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Biochemistry
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JoVE Journal Biochemistry
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
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分離とシロイヌナズナ由来のミトコンドリアの呼吸測定

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15,063 Views

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09:54 min

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January 05, 2018

DOI:

10.3791/56627-v

DOI:
10.3791/56627-v

•

09:54 min
January 05, 2018

•

14 Views
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1ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science,La Trobe University, 2School of Biological Sciences,Flinders University, 3ARC Centre of Excellence in Plant Energy Biology,University of Western Australia

Summary

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ミトコンドリアは、植物細胞のわずかな割合は、彼ら研究の範囲を精製する必要があります。ミトコンドリアは、均質化、続いて高純度ミトコンドリア分画を取得する微分と密度勾配遠心法によってさまざまな植物器官から分離できます。

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Cite this Article

Lyu, W., Selinski, J., Li, L., Day, D. A., Murcha, M. W., Whelan, J., Wang, Y. Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana. J. Vis. Exp. (131), e56627, doi:10.3791/56627 (2018).

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