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人类的过表达和纯化<em>顺</emβ-肾上腺素转移酶<em>大肠杆菌</em
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Immunology and Infection
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JoVE Journal Immunology and Infection
Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
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人类的过表达和纯化<em>顺</emβ-肾上腺素转移酶<em>大肠杆菌</em

DOI:
10.3791/56430-v

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07:35 min

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August 03, 2017

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, , , , , , , ,
1Sackler Faculty of Medicine,Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine,Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine,Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine,Tel Aviv University

Chapters

  • 00:05Title
  • 00:56Purification of Human DHDDS
  • 03:52Analytical Size-exclusion Chromatography (SEC)
  • 04:40Enzyme Kinetics – Time-dependent Activity
  • 06:15Results: Purification and Analysis of Human DHDDS
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

来自大肠杆菌的非变性条件下用于过表达和纯化密码子优化的人顺式 -丙烯基转移酶的简单方案 大肠杆菌 ，以及酶活性测定法。该方案可以推广用于生产适合于机械研究的数量和质量的其它顺式异戊烯基转移酶蛋白质。

Tags

Cis-prenyltransferaseHuman DHDDSOverexpressionPurificationEscherichia ColiIMACSize Exclusion ChromatographyTEV ProteaseRetinitis Pigmentosa

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