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活细胞 Sod1 突变蛋白聚集物高含量定量测定方法的建立
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JoVE Journal Neuroscience
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
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活细胞 Sod1 突变蛋白聚集物高含量定量测定方法的建立

DOI:
10.3791/56425-v

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11:12 min

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October 04, 2017

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, , ,
1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies,Institut Pasteur Korea, 2School of Pharmacy,Sungkyunkwan University, 3Technology Development Platform,Institut Pasteur Korea

Chapters

  • 00:05Title
  • 00:58Lentivirus Production
  • 02:50Lentiviral Transduction and Stable Line Generation
  • 04:53Dose Response Effect of Proteosome Inhibitors on Protein Aggregation and Image Analysis
  • 07:06Time lapse Imaging of YFP Expression in Living Cells
  • 08:09Results: Quantification of SOD1 Mutant Protein Aggregate Formation
  • 10:04Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述一种方法来量化错误蛋白质的聚集。我们的协议细节慢诱导稳定细胞线生成, 自动共焦成像, 和图像分析的蛋白质集料。作为一个说明性应用, 我们研究了小分子在促进 SOD1 聚集的时间和剂量依赖性的方式的影响。

Tags

Assay DevelopmentHigh Content QuantificationSod1 Mutant ProteinProtein Aggregate FormationLiving CellsNeurodegenerative DisordersALSLentivirus ProductionHEK-293 T-cellsTransfectionCell Transduction

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