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Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
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JoVE Journal Medicine
Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

DOI:
10.3791/56410-v

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08:32 min

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January 04, 2018

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,
1The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University and National Health Research, 2Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 3TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University

Chapters

  • 00:05Title
  • 01:21Preparation of Micro-osmotic Pump Infusion Systems
  • 02:41Intracerebral Ventricular Surgery – Implantation of the Micro-osmotic Pump
  • 04:15Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
  • 06:04Results: Glucose Metabolism and Insulin Signal Activation
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol studies the role of chemokine (C-C motif) ligand 5 (CCL5) in the hypothalamus by delivering an antagonist, MetCCL5, into the mouse brain using a micro-osmotic pump brain infusion system. This transient inhibition of CCL5 activity interrupted hypothalamic insulin signaling, leading to glucose intolerance and peripheral systemic insulin sensitivity.

Tags

Hypothalamic Insulin SignalPeripheral Glucose IntoleranceC-C-L-5 SignalingMet-C-C-L-5Micro-osmotic PumpArtificial Cerebrospinal FluidBrain Infusion KitStereotactic ApparatusAnesthetized MouseBrain Map

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