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Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
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January 04, 2018
DOI:
1The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University and National Health Research, 2Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 3TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Summary
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This protocol studies the role of chemokine (C-C motif) ligand 5 (CCL5) in the hypothalamus by delivering an antagonist, MetCCL5, into the mouse brain using a micro-osmotic pump brain infusion system. This transient inhibition of CCL5 activity interrupted hypothalamic insulin signaling, leading to glucose intolerance and peripheral systemic insulin sensitivity.
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Ajoy, R., Chou, S. Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain. J. Vis. Exp. (131), e56410, doi:10.3791/56410 (2018).
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