Journal
/
Biochemistry
/
Een alternatieve cultuur methode om Genomic Hypomethylation van muis embryonale stamcellen met MEK-remmer PD0325901 en vitamine C
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
Please note that all translations are automatically generated. Click here for the English version.

Een alternatieve cultuur methode om Genomic Hypomethylation van muis embryonale stamcellen met MEK-remmer PD0325901 en vitamine C

Nederlands

Automatically Generated

6,515 Views

•

11:53 min

•

June 01, 2018

DOI:

10.3791/56391-v

DOI:
10.3791/56391-v

•

11:53 min
June 01, 2018

•

1 Views
, ,
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences

Summary

Automatically generated

Wij beschreven twee chemische-gebaseerde protocollen voor het kweken van muis embryonale stamcellen in detail. Deze nieuwe methode maakt gebruik van synergetische mechanismen ter bevordering van Tet-gemedieerde oxidatie (door vitamine C) en onderdrukken van DOVO synthese van 5-methylcytosine (door PD0325901) om DNA hypomethylation en pluripotent van muis embryonale stamcellen te onderhouden.

Explore More Videos

Mouse Embryonic Stem CellsGenomic HypomethylationMEK Inhibitor PD0325901Vitamin CCulture MethodDNA ExtractionDNA MethylationPluripotencyHeterogeneityMorphologyUndifferentiated State

Read Article

Cite this Article

Li, C., Lai, W., Wang, H. An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C. J. Vis. Exp. (136), e56391, doi:10.3791/56391 (2018).

Download .ris file

Copy

Share Video

.

Copy