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En temps réel Quaking induite par le test de Conversion pour détection de CWD Prions dans les matières fécales
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
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En temps réel Quaking induite par le test de Conversion pour détection de CWD Prions dans les matières fécales

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13,346 Views

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09:50 min

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September 29, 2017

DOI:

10.3791/56373-v

DOI:
10.3791/56373-v

•

09:50 min
September 29, 2017

•

10 Views
, , , , ,
1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine,University of Calgary, 2Dept. of Molecular Biology,University of Wyoming, 3Canadian Food Inspection Agency,Lethbridge Laboratories

Summary

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Nous présentons ici un protocole pour décrire une technique d’amplification de prion simple, rapide et efficace, la méthode de conversion induite par le tremble en temps réel (RT-QuIC).

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Cite this Article

Cheng, Y. C., Hannaoui, S., John, T. R., Dudas, S., Czub, S., Gilch, S. Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material. J. Vis. Exp. (127), e56373, doi:10.3791/56373 (2017).

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