Dergi
/
İmmünoloji ve Enfeksiyon
/
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

13,334 Views

•

09:50 min

•

September 29, 2017

DOI:

10.3791/56373-v

DOI:
10.3791/56373-v

•

09:50 min
September 29, 2017

•

10 Views
, , , , ,
1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine,University of Calgary, 2Dept. of Molecular Biology,University of Wyoming, 3Canadian Food Inspection Agency,Lethbridge Laboratories

Özet

Automatically generated

Here, we present a protocol to describe a simple, fast and efficient prion amplification technique, the real-time quaking-induced conversion (RT-QuIC) method.

Explore More Videos

CWD PrionsFecal MaterialReal-time Quaking-induced Conversion AssayCervidChronic Wasting DiseaseSensitive DetectionHigh ThroughputSodium Phosphotungstic Acid PrecipitationRT-QuIC AssayThioflavin T

Read Article

Cite this Article

Cheng, Y. C., Hannaoui, S., John, T. R., Dudas, S., Czub, S., Gilch, S. Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material. J. Vis. Exp. (127), e56373, doi:10.3791/56373 (2017).

Download .ris file

Copy

Share Video

.

Copy