A streamlined protocol for performing an extensive biochemical and structural characterization of a carbohydrate substrate binding protein from Streptococcus pneumoniae is presented.
Culurgioni, S., Tang, M., Hall, D. R., Walsh, M. A. Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092. J. Vis. Exp. (128), e56294, doi:10.3791/56294 (2017).