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用于实时细胞分析仪的阵列金电极再生
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
DOI:

12:47 min

March 12, 2018

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Chapters

  • 00:04Title
  • 00:52Proliferation/Cytotoxicity Experiment and Data Acquisition
  • 03:54Case 1: Regeneration of Electronic Plates after Cytotoxicity Evaluation of Anti-cancer Drug
  • 05:53Case 2: Regeneration of Electronic Plates after Drug Delivery using Mesoporous Material
  • 07:34Assessment of Regeneration Effect
  • 10:22Results: Evaluationof Regenerated Electronic Plates
  • 12:01Conclusion

Summary

Automatic Translation

该协议描述了一个通用的战略, 以重新生成商业阵列金电极装备的无标签的细胞分析仪, 旨在节省高运行成本 ofmicrochip 的化验。再生过程包括胰蛋白酶消化, 用乙醇和水冲洗, 和一个纺纱步骤, 使微晶片的重复使用。

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