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Preparazione dei campioni e analisi dei dati di espressione genica basati su RNASeq dallo Zebrafish
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Genetics
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JoVE Journal Genetics
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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Preparazione dei campioni e analisi dei dati di espressione genica basati su RNASeq dallo Zebrafish

italiano

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10,476 Views

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11:42 min

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October 27, 2017

DOI:

10.3791/56187-v

DOI:
10.3791/56187-v

•

11:42 min
October 27, 2017

•

3 Views
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1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine,University of Maryland School of Medicine

Summary

Automatically generated

Questo protocollo presenta un approccio per l'analisi del trascrittoma intero da embrioni di zebrafish, larve, o ordinato cells. Includiamo isolamento di RNA, analisi dei percorsi dei dati RNASeq e convalida qRT-PCR-basata di cambiamenti di espressione genica.

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RNASeqGene ExpressionZebrafishEmbryoLarvaTranscriptomeSample PreparationRNA ExtractionRNA SequencingDevelopmental BiologyComparative Analysis

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Cite this Article

Hostelley, T. L., Nesmith, J. E., Zaghloul, N. A. Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish. J. Vis. Exp. (128), e56187, doi:10.3791/56187 (2017).

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