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Correlativa Super-resolução e microscopia eletrônica para resolver a localização da proteína na Retina de Zebrafish
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Correlativa Super-resolução e microscopia eletrônica para resolver a localização da proteína na Retina de Zebrafish

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9,164 Views

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12:28 min

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November 10, 2017

DOI:

10.3791/56113-v

DOI:
10.3791/56113-v

•

12:28 min
November 10, 2017

•

2 Views
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1Center for Microscopy and Image Analysis,University of Zurich, 2Institute for Molecular Life Sciences,University of Zurich, 3Institute for Medical Genetics,University of Zurich

Summary

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Este protocolo descreve as etapas necessárias para obter resultados de localização Subcellular da proteína na retina de zebrafish, correlacionando a microscopia de luz super resolução e imagens de microscopia eletrônica de varredura.

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Cite this Article

Mateos, J. M., Barmettler, G., Doehner, J., Ojeda Naharros, I., Guhl, B., Neuhauss, S. C., Kaech, A., Bachmann-Gagescu, R., Ziegler, U. Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina. J. Vis. Exp. (129), e56113, doi:10.3791/56113 (2017).

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