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Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

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12:28 min

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November 10, 2017

DOI:

10.3791/56113-v

DOI:
10.3791/56113-v

•

12:28 min
November 10, 2017

•

2 Views
, , , , , , , ,
1Center for Microscopy and Image Analysis,University of Zurich, 2Institute for Molecular Life Sciences,University of Zurich, 3Institute for Medical Genetics,University of Zurich

Summary

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This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images.

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Mateos, J. M., Barmettler, G., Doehner, J., Ojeda Naharros, I., Guhl, B., Neuhauss, S. C., Kaech, A., Bachmann-Gagescu, R., Ziegler, U. Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina. J. Vis. Exp. (129), e56113, doi:10.3791/56113 (2017).

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