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In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats
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JoVE Journal Neuroscience
In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats

In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats

DOI:
10.3791/56090-v

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08:19 min

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September 27, 2017

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, , , , ,
1Hongqiao International Institute of Medical Research, Tongren Hospital and Department of Physiology, Faculty of Basic Medical Sciences,Shanghai Jiaotong University School of Medicine

Chapters

  • 00:05Title
  • 00:58Tissue Collection
  • 02:50Dissection of the Colonic Afferent Nerves
  • 03:49Electrophysiological Recording and Testing Colonic Afferent Sensitivity
  • 06:09Electrophysiological Recording of Rat Colonic Efferent Nerves and Pelvic Nerves
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

Abnormal sensory function underlies visceral pain and other symptoms of functional and inflammatory bowel diseases. A protocol for the electrophysiological recording of the colonic afferent nerves in an ex vivo rat colorectum preparation is presented here.

Tags

Keywords: In VitroElectrophysiologyColonic Afferent FibersRatsPelvic GanglionColorectumKrebs SolutionIntraluminal InfusionNerve Dissection

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