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Journal
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Biochemistry
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氮气气蚀和差速离心允许监测外周血膜蛋白在细胞中的分布
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Biochemistry
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JoVE Journal
Biochemistry
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
Please note that all translations are automatically generated.
Click here for the English version.
氮气气蚀和差速离心允许监测外周血膜蛋白在细胞中的分布
DOI:
10.3791/56037-v
•
08:24 min
•
August 18, 2017
•
Mo Zhou
,
Mark R. Philips
1
Langone Medical Center
,
New York University
Chapters
00:05
Title
00:41
Cell Harvesting
01:35
Nitrogen Cavitation
03:31
Separation of Cytosolic and Membrane Fractions
05:22
Results: Partitioning of Cellular Proteins in Membrane and Cytosolic Fractions
07:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里我们目前用于洗涤剂免费培养的哺乳动物细胞基于氮空和随后的分离,胞浆和膜结合蛋白的超速离心同质化的协议。此方法是理想的监测分区之间可溶性的外周膜蛋白和膜组分。
Tags
Nitrogen Cavitation
Differential Centrifugation
Peripheral Membrane Proteins
Cultured Cells
Subcellular Compartmentalization
HEK293 Cells
Homogenization Buffer
Pressurization
Protease Inhibitor
Cell Disruption
Fractionation
Soluble Fraction
Membrane Fraction
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