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Rening av membran facket för endoplasmatiska nätverket-associerade nedbrytningen av exogena antigen i Cross-presentation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
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Rening av membran facket för endoplasmatiska nätverket-associerade nedbrytningen av exogena antigen i Cross-presentation

8,118 Views

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12:48 min

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August 21, 2017

DOI:

10.3791/55949-v

DOI:
10.3791/55949-v

•

12:48 min
August 21, 2017

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3 Views
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1Laboratory of Physiological Chemistry, Faculty of Pharmacy,Takasaki University of Health and Welfare

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Membrane CompartmentEndoplasmic Reticulum-associated Degradation (ERAD)Cross-presentationExogenous AntigenDendritic CellsBiotinylated Ovalbumin (bOVA)Vesicle IsolationMicrosomeUbiquitination

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Imai, J., Otani, M., Sakai, T., Hatta, S. Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation. J. Vis. Exp. (126), e55949, doi:10.3791/55949 (2017).

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