The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.
Imai, J., Otani, M., Sakai, T., Hatta, S. Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation. J. Vis. Exp. (126), e55949, doi:10.3791/55949 (2017).