Journal
/
免疫与感染
/
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

DOI:
10.3791/55949-v

•

12:48 min

•

August 21, 2017

•

, , ,
1Laboratory of Physiological Chemistry, Faculty of Pharmacy,Takasaki University of Health and Welfare

Chapters

  • 00:05Title
  • 01:13Preparation of Microsomes
  • 04:44Purification of Microsomes with bOVA Underground ERAD
  • 06:59In Vitro Reconstruction of ERAD Ubiquination of bOVA Using Purified Microsomes
  • 09:04Results: Purified Microsomes and In Vitro Ubiquination of the Exogenous Antigen
  • 11:06Conclusion

Summary

自动翻译

自动翻译

The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.

Tags

Membrane CompartmentEndoplasmic Reticulum-associated Degradation (ERAD)Cross-presentationExogenous AntigenDendritic CellsBiotinylated Ovalbumin (bOVA)Vesicle IsolationMicrosomeUbiquitination

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells

Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

Assembly and Purification of Prototype Foamy Virus Intasomes

Read Article