This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices.
Weng, W., Li, D., Peng, C., Behnisch, T. Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System. J. Vis. Exp. (131), e55936, doi:10.3791/55936 (2018).