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Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
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Biochemistry
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JoVE Journal Biochemistry
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

8,808 Views

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12:55 min

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October 10, 2017

DOI:

10.3791/55932-v

DOI:
10.3791/55932-v

•

12:55 min
October 10, 2017

•

1 Views
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1Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Tags

Tau PhosphorylationMicrotubule BindingTau LocalizationConfocal MicroscopyColorectal Cancer CellsPhosphatase AssayMicrotubule-binding AssayTau ProteinHCT 116 CellsCurcuminLithium ChlorideRIPA BufferBradford AssayAlkaline Phosphatase

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Huda, M. N., Erdene-Ochir, E., Pan, C. Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells. J. Vis. Exp. (128), e55932, doi:10.3791/55932 (2017).

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