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Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
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Biochemistry
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JoVE Journal Biochemistry
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

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8,832 Views

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12:55 min

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October 10, 2017

DOI:

10.3791/55932-v

DOI:
10.3791/55932-v

•

12:55 min
October 10, 2017

•

2 Views
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1Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Summary

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This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.

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Huda, M. N., Erdene-Ochir, E., Pan, C. Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells. J. Vis. Exp. (128), e55932, doi:10.3791/55932 (2017).

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