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通过机械组织分离和流式细胞术对哺乳动物雄性生殖细胞多特异性纯化的标准化方法
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
DOI:

08:46 min

July 12, 2017

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Chapters

  • 00:05Title
  • 01:10Testicular Tissue Single Cell Suspension Generation
  • 02:26Cell Staining and Fluorescence Activiated Cell Sorting (FACS)
  • 05:33Results: Representative Testicular Cell Subset Analyses
  • 07:17Conclusion

Summary

Automatic Translation

这项工作描述了从不同哺乳动物物种的睾丸组织获得纯化的生殖细胞群体的方法的标准化。结合机械睾丸解离,染色与Hoechst-33342和碘化丙啶和FACS分选是一个简单的方案,广泛应用于男性生殖生物学的比较研究。

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