Journal
/
Genetics
/
Chromatine Immunoprecipitatie (ChIP) in Muis T-cellijnen
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
Please note that all translations are automatically generated. Click here for the English version.

Chromatine Immunoprecipitatie (ChIP) in Muis T-cellijnen

Nederlands

Automatically Generated

17,875 Views

•

11:39 min

•

June 17, 2017

DOI:

10.3791/55907-v

DOI:
10.3791/55907-v

•

11:39 min
June 17, 2017

•

8 Views
, ,
1Institute of Biochemistry,University of Giessen

Summary

Automatically generated

Dit werk beschrijft een protocol voor chromatine immunoprecipitatie (ChIP) met behulp van een volwassen T-cellijn van de muis. Dit protocol is geschikt om de verdeling van specifieke histonenmerken op specifieke promotor sites of genoombreed te onderzoeken.

Explore More Videos

Chromatin ImmunoprecipitationChIPMouse T-cell LinesHistone MarksChromatin BiologyChromatin ShearingFormaldehyde CrosslinkingGlycine QuenchingSonicationFocused UltrasonicatorLysis BufferCell PelletSupernatant

Read Article

Cite this Article

Giaimo, B. D., Ferrante, F., Borggrefe, T. Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines. J. Vis. Exp. (124), e55907, doi:10.3791/55907 (2017).

Download .ris file

Copy

Share Video

.

Copy